When can I consider an epitope as non-binder using MHC class I and II binding predictions tools?

Originally from ticket #1739.

Question:

I would appreciate if you can explain me more detailed when I can consider an epitope as non-binder using MHC class I and II binding predictions tools.

Answer:

Thank you for your inquiry. Please see the article under the Prediction Tool topic (http://help.iedb.org/entries/23854373-Selecting-thresholds-cut-offs-for-MHC-class-I-and-II-binding-predictions). I also suggest watching 2 webcast presentations from the 2014 IEDB User Workshop (http://help.iedb.org/entries/140865-How-To-Videos) on the MHC Class I and II prediction tools. Your question is addressed in both presentations in greater detail. The slides are available at http://help.iedb.org/entries/58903209-2014-IEDB-User-Workshop.